Submitted by Zara Daniela on
The final session of the day in Assen ran in excellent conditions, which meant that riders were quick to improve their times from FP1, Pecco Bagnaia once again being the fastest man on track. Joan Mir caught up to trail the Italian by only one tenth of a second, sandwiched between the two Sky riders, Luca Marini having a bit off a wild ride into third position.
Fabio Quartararo continued to impress up in fourth, less than two tenths separating him from the lead of the session and with Lorenzo Baldassarri also finding some pace another tenth behind. The Italian made a late jump into the top five and brought compatriot Mattia Pasini with him. The two Italians were closely followed by Alex Marquez, who took his time to find improvements following his morning crash. Only a thousandth of a second behind finished Marcel Schrotter in eighth, with Romano Fenati and Miguel Oliveira completing the top ten within half a second of Bagnaia.
Sam Lowes narrowly missed out on the top ten, followed by Andrea Locatelli, Danny Kent and Xavi Vierge, while Jorge Navarro had a difficult afternoon finishing in 18th position but keeping in mind that the top 20 riders were covered by one second.
Results:
| Pos. | Num. | Rider | Bike | Time | Gap 1st | Prev. |
| 1 | 42 | Francesco BAGNAIA | Kalex | 1'38.091 | ||
| 2 | 36 | Joan MIR | Kalex | 1'38.191 | 0.100 | 0.100 |
| 3 | 10 | Luca MARINI | Kalex | 1'38.246 | 0.155 | 0.055 |
| 4 | 20 | Fabio QUARTARARO | Speed Up | 1'38.256 | 0.165 | 0.010 |
| 5 | 7 | Lorenzo BALDASSARRI | Kalex | 1'38.371 | 0.280 | 0.115 |
| 6 | 54 | Mattia PASINI | Kalex | 1'38.386 | 0.295 | 0.015 |
| 7 | 73 | Alex MARQUEZ | Kalex | 1'38.525 | 0.434 | 0.139 |
| 8 | 23 | Marcel SCHROTTER | Kalex | 1'38.526 | 0.435 | 0.001 |
| 9 | 13 | Romano FENATI | Kalex | 1'38.565 | 0.474 | 0.039 |
| 10 | 44 | Miguel OLIVEIRA | KTM | 1'38.579 | 0.488 | 0.014 |
| 11 | 22 | Sam LOWES | KTM | 1'38.612 | 0.521 | 0.033 |
| 12 | 5 | Andrea LOCATELLI | Kalex | 1'38.637 | 0.546 | 0.025 |
| 13 | 52 | Danny KENT | Speed Up | 1'38.749 | 0.658 | 0.112 |
| 14 | 97 | Xavi VIERGE | Kalex | 1'38.801 | 0.710 | 0.052 |
| 15 | 40 | Augusto FERNANDEZ | Kalex | 1'38.871 | 0.780 | 0.070 |
| 16 | 89 | Khairul Idham PAWI | Kalex | 1'38.887 | 0.796 | 0.016 |
| 17 | 41 | Brad BINDER | KTM | 1'38.893 | 0.802 | 0.006 |
| 18 | 9 | Jorge NAVARRO | Kalex | 1'38.903 | 0.812 | 0.010 |
| 19 | 24 | Simone CORSI | Kalex | 1'38.933 | 0.842 | 0.030 |
| 20 | 32 | Isaac VIÑALES | Kalex | 1'39.057 | 0.966 | 0.124 |
| 21 | 87 | Remy GARDNER | Tech 3 | 1'39.107 | 1.016 | 0.050 |
| 22 | 64 | Bo BENDSNEYDER | Tech 3 | 1'39.182 | 1.091 | 0.075 |
| 23 | 27 | Iker LECUONA | KTM | 1'39.197 | 1.106 | 0.015 |
| 24 | 45 | Tetsuta NAGASHIMA | Kalex | 1'39.340 | 1.249 | 0.143 |
| 25 | 77 | Dominique AEGERTER | KTM | 1'39.495 | 1.404 | 0.155 |
| 26 | 4 | Steven ODENDAAL | NTS | 1'39.525 | 1.434 | 0.030 |
| 27 | 66 | Niki TUULI | Kalex | 1'39.659 | 1.568 | 0.134 |
| 28 | 16 | Joe ROBERTS | NTS | 1'39.779 | 1.688 | 0.120 |
| 29 | 62 | Stefano MANZI | Suter | 1'39.905 | 1.814 | 0.126 |
| 30 | 51 | Eric GRANADO | Suter | 1'40.405 | 2.314 | 0.500 |
| 31 | 95 | Jules DANILO | Kalex | 1'40.872 | 2.781 | 0.467 |
| 32 | 18 | Xavi CARDELUS | Kalex | 1'41.106 | 3.015 | 0.234 |
| 33 | 21 | Federico FULIGNI | Kalex | 1'41.770 | 3.679 | 0.664 |
